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ID | Components | Size |
EP139-C01 | High-bind Plate | 1plate |
EP139-C02 | Human IL-17RA | 15μg |
EP139-C03 | Biotinylated Human IL-17A | 10μg |
EP139-C04 | Anti-IL-17A Neutralizing Antibody | 20μg |
EP139-C05 | Streptavidin-HRP | 10μg |
EP139-C06 | Coating Buffer | 12mL |
EP139-C07 | 10xWashing Buffer | 50mL |
EP139-C08 | Blocking Buffer | 50mL |
EP139-C09 | Substrate Solution | 12mL |
EP139-C10 | Stop Solution | 7mL |
This kit is developed for screening for inhibitors of human IL-17A binding to human IL-17RA.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-17 RA.
b) Add your molecule of interest to the tests.
c) Add human IL-17A-Biotin to bind the coated IL-17 RA.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-17A: IL-17 RA binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF IL-17A[Biotinylated]: IL-17RA BINDING BY ANTI-IL-17A NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-17A Neutralizing antibody (Catalog # EP139-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-17A: IL-17RA binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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