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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
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| Assay Type | Sandwich-ELISA |
| Analyte | SCF |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | ＜15.625 pg/mL |
| Standard Curve Range | 15.625 pg/mL-1000 pg/mL |
| Assay Time | 2 hr 50 min |
| Suitable Sample Type | For the detection and quantitative determination of GMP human SCF in human serum and cell culture supernates. |
| Sample volume | 100 uL |
The kit is developed for the detection and quantitative determination of GMP human SCF in human serum and cell culture supernates.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES006-C01 | Pre-coated Anti-SCF Antibody Microplate | 1 plate |
| RES006-C02 | Human SCF Standard | 20 μg |
| RES006-C03 | Biotin-Anti-SCF Antibody | 20 μg |
| RES006-C04 | Streptavidin-HRP | 50 μL |
| RES006-C05 | 10×Washing Buffer | 50 mL |
| RES006-C06 | 2×Dilution Buffer | 50 mL |
| RES006-C07 | Substrate Solution | 12 mL |
| RES006-C08 | Stop Solution | 7 mL |
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human SCF as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-SCF Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of human SCF were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Five parts of blank T cell culture supernatant were added with different concentrations of human SCF, and the T cell culture supernatant without human SCF was used as background to calculate the recovery rate. The range of the recovery rate is 90.9-101.3%, and the average recovery is 95.3%.
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