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Your Position: Accueil > Protein > NME1 > NM1-H5147

Human NME1 / NDKA / NM23-H1 Protein, His Tag

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  • Synonym
    NME1,NDP kinase A,NDPA,GAAD,NM23-H1
  • Source
    Human NME1, His Tag(NM1-H5147) is expressed from E. coli cells. It contains AA Ala 2 - Glu 152 (Accession # AAH00293).
    Predicted N-terminus: Met
  • Molecular Characterization
    NME1 Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 18.0 kDa. The protein migrates as 17-18 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NME1 SDS-PAGE

Human NME1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%.

  • Background
    Nucleoside diphosphate kinase A, a member of the NDK family, is also known as NME1, NDP kinase A (NDPA), granzyme A-activated DNase (GAAD), metastasis inhibition factor nm23 (NM23-H1) and tumor metastatic process-associated protein. NME1 plays a major role in the synthesis of nucleoside triphosphates other than ATP. NME1 is also involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Furthermore, NME1 is required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, NME1 works in concert with TREX1. NME1 nicks one strand of D and TREX1 removes bases from the free 3 end to enhance D damage and prevent D end reannealing and rapid repair.
  • Clinical and Translational Updates

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