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Your Position: Accueil > Protein > B7-H4 > B74-H8222

Biotinylated Human B7-H4 Protein, His Tag, ultra sensitivity (primary amine labeling) (MALS verified)

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  • Synonym
    B7-H4,VTCN1,B7S1,B7h.5
  • Source
    MABSol® Biotinylated Human B7-H4, His Tag, primary amine labeling (B74-H8222) is expressed from human HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902). It is the biotinylated form of Human B7-H4, His Tag (Cat. No. B74-H5222).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    B7-H4 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.4 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H4 SDS-PAGE

Biotinylated Human B7-H4, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
B7-H4 MALS images

The purity of Biotinylated Human B7-H4, His Tag, primary amine labeling (Cat. No. B74-H8222) is more than 85% and the molecular weight of this protein is around 48-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 B7-H4 ELISA

Immobilized Anti-Human B7-H4 mAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag, primary amine labeling (Cat. No. B74-H8222) with a linear range of 1-8 ng/mL (QC tested).

  • Background
    V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €290.00

Price(EUR) : €1390.00

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