0
Il n'y a pas de produit dans le panier !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Accueil > Protein > EGF R > EGR-H522a

Human EGF R Protein, His Tag, low endotoxin (MALS verified)

Order Now

  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Human EGF R, His Tag, low endotoxin(EGR-H522a) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    EGF R Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5kDa. The protein migrates as 77-104 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
EGF R SDS-PAGE

Human EGF R, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
EGF R MALS images

The purity of Human EGF R, His Tag, low endotoxin (Cat. No. EGR-H522a) is more than 90% and the molecular weight of this protein is around 77-104 kDa verified by SEC-MALS.

Bioactivity-ELISA
 EGF R ELISA

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag, low endotoxin (Cat. No. EGR-H522a) with a linear range of 1.6-13 ng/mL (QC tested).

 EGF R ELISA

Immobilized Human EGF R, His Tag, low endotoxin (Cat. No. EGR-H522a) at 1 μg/mL (100 μL/well) can bind Anti-EGFR Peptide with a linear range of 0.06-2 ng/mL (Routinely tested).

Bioactivity-SPR
 EGF R SPR

Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R, His Tag, low endotoxin (Cat.No. EGR-H522a) with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).

 EGF R SPR

Human EGF R, His Tag, low endotoxin (Cat. No. EGR-H522a) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.76 nM as determined in SPR assay (Biacore T200) (Routinely tested).

  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
  • Clinical and Translational Updates

Comments (0)


ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €510.00

Price(EUR) : €2670.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:31 Details
  • Number of Drugs in Clinical Trials:169 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop

Laisser un message