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Your Position: Accueil > Protein > IL-15 R alpha > ILA-H82F4

Biotinylated Human IL-15 R alpha / CD215 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha
  • Source
    Biotinylated Human IL-15 R alpha, Fc,Avitag(ILA-H82F4) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1).
    Predicted N-terminus: Ile 31
  • Molecular Characterization
    IL-15 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 46.6 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-15 R alpha SDS-PAGE

Biotinylated Human IL-15 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-15 R alpha MALS images

The purity of Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-15 R alpha ELISA

Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 2-39 ng/mL (QC tested).

 IL-15 R alpha ELISA

Immobilized Mouse IL-15, Fc Tag (Cat. No. IL5-M5255) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 10-156 ng/mL (Routinely tested).

  • Background
    IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
  • Clinical and Translational Updates

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