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Your Position: Accueil > Protein > Complement C5 > CO5-C52Hx

Cynomolgus Complement C5 Protein, His Tag

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  • Synonym
    Complement C5,C5,CPAMD4
  • Source
    Cynomolgus Complement C5, His Tag(CO5-C52Hx) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # XP_005580972.1).
    Predicted N-terminus: Gln 19 (β chain) & Met 678 (α chain)
  • Molecular Characterization
    Complement C5 Structure

    This protein carries a polyhistidine tag at the C-terminus.The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 65 kDa and 113 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Complement C5 SDS-PAGE

Cynomolgus Complement C5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Complement C5 ELISA

Immobilized Cynomolgus Complement C5, His Tag (Cat. No. CO5-C52Hx) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

 Complement C5 ELISA

Immobilized Cynomolgus Complement C5, His Tag (Cat. No. CO5-C52Hx) at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.5-31 ng/mL (Routinely tested).

  • Background
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2570.00

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