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This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 74.1 kDa. The protein migrates as 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD27 Ligand Protein, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266) is more than 85% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.
Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266) with a linear range of 0.2-25 ng/mL (QC tested).
Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266): Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.74034 μg/mL (Routinely tested).
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