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Your Position: Accueil > Protein > CD27 Ligand > CDL-H82D7

Biotinylated Human CD27 Ligand / CD70 Protein, His,Avitag™,Flag Tag, active trimer (MALS verified)

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  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (CDL-H82D7) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1 ) trimer Design.
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag. The Avi tag (Avitag™) is inserted in-between the polyhistidine tag and flag tag.

    The protein has a calculated MW of 51.0 kDa. The protein migrates as 47-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD27 Ligand SDS-PAGE

Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD27 Ligand MALS images

The purity of Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD27 Ligand ELISA

Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) with a linear range of 0.1-4 ng/mL (QC tested).

 CD27 Ligand ELISA

Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27, Fc Tag (Cat. No. CD7-H5254) : Biotinylated Human CD27 Ligand, His,Avitag,Flag Tag (Cat. No. CDL-H82D7) binding reactions. The half maximal inhibitory concentration (IC50) is 3.05858 μg/mL (Routinely tested).

  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €1540.00

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