0
Il n'y a pas de produit dans le panier !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Accueil > Beads > CD3E & CD3D > MBS-K003

Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

Order Now

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈1531 pmol (49 μg) CD3E & CD3D/mg Beads≈1531 pmol (49 μg) CD3E & CD3D/mg Beads
Binding Capacity>267 pmol (40 μg) antibody/mg beads>267 pmol (40 μg) antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The biotinylated CD3E & CD3D protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD3E & CD3D antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human CD3E & CD3D coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
  • Source
    Human CD3E & CD3D Heterodimer protein is expressed from human 293 cells (HEK293).It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
    *The Isotype control (Cat. No. SMB-B01) is sold separately.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the lyophilized beads at -20°C for long term storage. The shelf life is 1 year at -20°C in lyophilized form.Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

Typical Data
 CD3E & CD3D TYPICAL DATA

Add CD3 pre-coupling magnetic beads into each well at a concentration of 1 mg/ml. 0.1 mg beads was added to each well. Then add increasing concentrations of anti-CD3 antibody to different wells. PE-labeled anti-human IgG Fc antibody was used for detection.

 CD3E & CD3D TYPICAL DATA

The binding curves between CD3E&D pre-coupling magnetic beads (Cat. No. MBS-K003) after different freeze-thaw cycles and anti-CD3 antibody. 0.1 mg of Beads (1 mg/mL, 100 μL) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/mL-0.039 μg/mL) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).

  • Clinical and Translational Updates

Comments (0)


ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €810.00

Price(EUR) : €2530.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop

Laisser un message