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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.0 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human NOTCH1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) is more than 85% and the molecular weight of this protein is around 170-210 kDa verified by SEC-MALS.
Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 5 μg/mL (100 μL/well) can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with a linear range of 1-8 ng/mL (QC tested).
Human Jagged 1, His Tag (Cat. No. JA1-H52H9) immobilized on CM5 Chip can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with an affinity constant of 5.13 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 67.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL1 Protein, His Tag, premium grade (Cat. No. DL1-H52H8) with an affinity constant of 290 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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