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Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag is produced by co-expression of CD3E and CD3D, has a calculated MW of 45.1 kDa (CD3E) & 42.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the polyhistidine tag is inserted in-between the Fc and Avi tags at the C-terminus and subunit CD3D contains a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the flag tag is inserted in-between the Fc and Avi tags at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 50-60 kDa under reducing (R) condition.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) is more than 90% and the molecular weight of this protein is around 95-110 kDa verified by SEC-MALS.
Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.4-3 ng/mL (QC tested).
Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind UCHT1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind Bispecific CD3×BCMA T cell-engaging Antibody with an affinity constant of 1.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind UCHT1 with an affinity constant of 21.3 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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Price(EUR) : €230.00
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