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Your Position: Accueil > Protein > CEACAM-5 > CE5-HF254

FITC-Labeled Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)

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  • Synonym
    CEACAM-5,CD66e,CEA,Meconium antigen 100
  • Source
    FITC-Labeled Human CEACAM-5, His Tag (CE5-HF254) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731-1). It is the FITC labeled form of Human CEACAM-5, His Tag (Cat. No.CE5-H5226).
    Predicted N-terminus: Lys 35
  • Molecular Characterization
    CEACAM-5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 72.2kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CEACAM-5 SDS-PAGE

FITC-Labeled Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
CEACAM-5 MALS images

The purity of FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CEACAM-5 ELISA

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) with a linear range of 0.039-0.625 μg/mL (QC tested).

  • Background
    Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
  • Clinical and Translational Updates

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Price(EUR) : €320.00

Price(EUR) : €2200.00

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