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Limited Edition Golden Llama is here! Check out how you can get one. 
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Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100mM Glycine, 25mM Arginine, 150mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-M52W2) is more than 90% and the molecular weight of this protein is around 95-110 kDa verified by SEC-MALS.
Immobilized Biotinylated CD3e Monoclonal Antibody at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate, can bind Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-M52W2) with a linear range of 0.016-0.25 μg/mL (QC tested).
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