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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (Cat. No. SPD-C5258) is more than 90% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.
Immobilized SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (Cat. No. SPD-C5258) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (Cat. No. SPD-C5258) with a linear range of 0.1-0.8 ng/mL (Routinely tested).
Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5258) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.05nM as determined in BLI assay (ForteBio Octet Red96e)(Routinely tested).
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
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