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| ID | Components | Size |
| RAS010-C01 | Pre-coated Anti-SARS-CoV-2 Nucleocapsid Antibody Microplate | 1 plate |
| RAS010-C02 | SARS-CoV-2 Nucleocapsid Protein | 10 ug |
| RAS010-C03 | Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody | 100 μL |
| RAS010-C04 | Streptavidin-HRP | 100 μL |
| RAS010-C05 | 10xWashing Buffer | 50 mL |
| RAS010-C06 | Dilution Buffer | 50 mL |
| RAS010-C07 | Substrate Solution | 12 mL |
| RAS010-C08 | Stop Solution | 7 mL |
This kit is developed for detecting the SARS-CoV-2 Nucleocapsid Protein in cell supernatant or SARS-CoV-2 virus in vitro.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.
b) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.
c) Add a diluted Streptavidin-HRP to the plate.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of SARS-CoV-2 Nucleocapsid Protein by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Protein Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein and Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody. Detection was performed using Streptavidin-HRP with sensitivity of 12.5 pg/mL (QC tested).
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