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| ID | Components | Size |
| RAS027-C01 | Pre-coated SARS-CoV-2 Spike Trimer(B.1.1.7) Microplate | 1 plate |
| RAS027-C02 | Positive Control | 100 μL |
| RAS027-C03 | Negative Control | 100 μL |
| RAS027-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS027-C05 | 10xWashing Buffer | 50 mL |
| RAS027-C06 | Dilution Buffer | 50 mL |
| RAS027-C07 | Substrate Solution | 12 mL |
| RAS027-C08 | Stop Solution | 7 mL |
This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike antibody in serum/plasma in vitro. The Spike antigen used in this kit contains 9 mutations (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) found in the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01).
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Trimer at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG in 1:800 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 78 ng/mL (QC tested).
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
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50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
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