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Your Position: Accueil > Protein > CD47 > CD7-H82A3

Biotinylated Human CD47 Protein, Mouse IgG2a Fc,Avitag™ (MALS verified)

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  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Biotinylated Human CD47, Mouse IgG2a Fc,Avitag(CD7-H82A3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    CD47 Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD47 SDS-PAGE

Biotinylated Human CD47, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD47 MALS images

The purity of Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) is more than 90% and the molecular weight of this protein is around 105-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD47 ELISA

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) with a linear range of 5-20 ng/mL (QC tested).

 CD47 ELISA

Immobilized Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD47 Antibody, Human IgG4 with a linear range of 1-10 ng/mL (Routinely tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • Clinical and Translational Updates

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