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Your Position: Accueil > Protein > Spike NTD > S1D-C52Hf

SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)

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  • Synonym
    S1 protein NTD,Spike protein S1 NTD,BetaCoV S1-NTD
  • Source
    SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (S1D-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (T95I, G142D, E154K)). The mutations (T95I, G142D, E154K) were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).
    Predicted N-terminus: Ser 13
  • Molecular Characterization
    Spike NTD Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike NTD SDS-PAGE

SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike NTD MALS images

The purity of SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (Cat. No. S1D-C52Hf) is more than 85% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike NTD ELISA

Immobilized SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (Cat. No. S1D-C52Hf) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-1 ng/mL (QC tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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Price(EUR) : €400.00

Price(EUR) : €2890.00

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