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Your Position: Accueil > Protein > PSCA > PSA-M82E3

Biotinylated Mouse PSCA Protein, His,Avitag™ (MALS verified)

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  • Synonyme
    PSCA,UNQ206,PRO232
  • Source
    Biotinylated Mouse PSCA, His,Avitag (PSA-M82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Asn 95 (Accession # P57096-1).
    Predicted N-terminus: Leu 21
  • Caractérisation moléculaire
    Online(Leu 21 - Asn 95) P57096-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 12.0 kDa. The protein migrates as 22-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxine
    Less than 0.1 EU per μg by the LAL method.
  • Pureté

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Stockage:

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse PSCA, His,Avitag (Cat. No. PSA-M82E3) SDS-PAGE gel

Biotinylated Mouse PSCA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Mouse PSCA, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse PSCA, His,Avitag (Cat. No. PSA-M82E3) is more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

  • CONTEXTE
    The Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI)-anchored protein, plays an important role in tumorigenesis. The prostate stem cell antigen (PSCA) gene, which encodes a prostate-specific antigen (PSA), was identified as a gene involved in cell adhesion and proliferation. PSCA may be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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