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Human GSK-3beta / GSK-3β Protein, His Tag (active enzyme, HPLC verified)

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  • Synonym
    GSK3B,Glycogen synthase kinase-3 beta
  • Source
    Human GSK-3beta, His Tag(GSB-H5545) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Thr 420 (Accession # P49841-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    GSK-3beta Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 48.8 kDa. The protein migrates as 49-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
GSK-3beta SDS-PAGE

Human GSK-3beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC
GSK-3beta SEC-HPLC

The purity of Human GSK-3beta, His Tag (Cat. No. GSB-H5545) was greater than 90% as determined by SEC-HPLC.

Bioactivity

The specific activity of GSK-3beta was determined to be >120 pmol/min/ug by ADP-Glo™ Kinase Assay using a synthetic substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) (QC tested).

  • Background
    Glycogen synthase kinase-3 (GSK-3) is a pivotal molecule in the development of Alzheimer's disease (AD). GSK-3beta is involved in the formation of paired helical filament (PHF)-tau, which is an integral component of the neurofibrillary tangle (NFT) deposits that disrupt neuronal function, and a marker of neurodegeneration in AD.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €380.00

Price(EUR) : €3450.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Phase 3 Clinical

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