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Human DDC / Dopa Decarboxilase Protein, His Tag (MALS verified)

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  • Synonym
    Aromatic-L-amino-acid decarboxylase,Dopa decarboxilase,AADC
  • Source
    Human DDC, His Tag(DDC-H55H6) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Glu 480 (Accession # P20711-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    DDC Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.8 kDa. The protein migrates as 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
DDC SDS-PAGE

Human DDC, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
DDC MALS images

The purity of Human DDC, His Tag (Cat. No. DDC-H55H6) is more than 90% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to convert the substrate 3, 4­dihydroxy L­phenylalanine (L­Dopa) to 3, 4­dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmol/min/µg (QC tested).

  • Background
    Dopa decarboxilase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase. The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. DDC inhibitors is in clinical use to treat Parkinson's disease(PD), usually co-administrated with L-DOPA. DDC would block peripheral conversion to dopamine and allow a greater percentage of L-DOPA to reach the brain, causing an increase in brain dopamine levels, and reducing the side effects of dopamine-rich blood.
  • Clinical and Translational Updates

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Price(EUR) : €240.00

Price(EUR) : €2180.00

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Drug Development Status

  • Number of Launched Drugs:8 Details
  • Number of Drugs in Clinical Trials:9 Details
  • Latest Research Phase:Approved

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