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Human Tau-441 / 2N4R (P301S) Protein, His Tag (MALS verified)

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  • Synonym
    DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU
  • Source
    Human Tau-441 (P301S), His Tag(TAU-H5145) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8 (P301S)).
    Predicted N-terminus: Met
  • Molecular Characterization
    Tau Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 47.7 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Tau SDS-PAGE

Human Tau-441 (P301S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Tau MALS images

The purity of Human Tau-441 (P301S), His Tag (Cat. No. TAU-H5145) is more than 85% and the molecular weight of this protein is around 53-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Tau ELISA

Immobilized Human Tau-441 (P301S), His Tag (Cat. No. TAU-H5145) at 1 μg/mL (100 μL/well) can bind Tau Monoclonal Antibody with a linear range of 0.4-13 ng/mL (QC tested).

  • Background
    Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimers disease, frontotemporal dementia, and other tauopathies.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2330.00

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