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Your Position: Accueil > Protein > IL-1RL1 > IL1-H82F8

Biotinylated Human IL-1RL1 / ST2 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1
  • Source
    Biotinylated Human IL-1RL1, Fc,Avitag(IL1-H82F8) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # Q01638-1).
    Predicted N-terminus: Lys 19
  • Molecular Characterization
    IL-1RL1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 62.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1RL1 SDS-PAGE

Biotinylated Human IL-1RL1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-1RL1 MALS images

The purity of Biotinylated Human IL-1RL1, Fc,Avitag (Cat. No. IL1-H82F8) is more than 90% and the molecular weight of this protein is around 165-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-1RL1 ELISA

Immobilized Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-1RL1, Fc,Avitag (Cat. No. IL1-H82F8) with a linear range of 0.6-10 ng/mL (QC tested).

  • Background
    Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €1390.00

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  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:NDA/BLA

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