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Your Position: Accueil > Protein > Plasma Kallikrein/KLKB1 > KL1-H52H9

Human Plasma Kallikrein / KLKB1 Protein, His Tag (active enzyme, MALS verified)

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  • Synonym
    Fletcher factor,kallikrein B, plasma (Fletcher factor) 1,kininogenin,KLK3 plasma kallikrein,KLKB1,plasma kallikrein heavy chain,plasma kallikrein light chain,Plasma Kallikrein,Plasma Prekallikrein,PPK,KLKB1
  • Source
    Human Plasma Kallikrein/KLKB1, His Tag(KL1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Ala 638 (Accession # P03952-1).
    Predicted N-terminus: Gly 20
  • Molecular Characterization
    Plasma Kallikrein/KLKB1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 71.1 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50mM Tris, 150mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Plasma Kallikrein/KLKB1 SDS-PAGE

Human Plasma Kallikrein/KLKB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Plasma Kallikrein/KLKB1 MALS images

The purity of Human Plasma Kallikrein/KLKB1, His Tag (Cat. No. KL1-H52H9) is more than 90% and the molecular weight of this protein is around 75-90 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1000 pmol/min/μg(QC tested).

  • Background
    Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Clinical and Translational Updates

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