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Your Position: Accueil > Protein > HLA-C*07:02:01 & B2M & RYR > HLR-H52W2

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-C*07:02:01 & B2M & RYR
  • Source
    Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein(HLR-H52W2) is expressed from human 293 cells (HEK293). It contains AA Cys 25 - Ile 308 (HLA-C*07:02:01) & Ile 21 - Met 119 (B2M) & RYRPGTVAL peptide (Accession # P10321 (HLA-C*07:02:01) & P61769 (B2M) & RYRPGTVAL).
    Predicted N-terminus: Cys 25 & Arg
  • Molecular Characterization

    Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein is produced by co-expression of HLA and B2M loaded with RYR peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.1 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-C*07:02:01 & B2M & RYR SDS-PAGE

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
 HLA-C*07:02:01 & B2M & RYR SPR

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer) (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) with an affinity constant of 2.40 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 HLA-C*07:02:01 & B2M & RYR SPR

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer) (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL2, Fc Tag (Cat. No. KI2-H5255) with an affinity constant of 1.01 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    HLA-A, B, and C are transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. The C receptor is a heterodimer consisting of a HLA-C mature gene product (heavy chain) and β2-microglobulin (light chain). The mature C chain is anchored in the membrane. HLA class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. HLA-C are expressed in nearly all cells, and present small peptides to the immune system which surveys for non-self peptides.Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein is a complex of HLA-C*07:02:01 of the MHC Class I, B2M and RMFPNAPYL peptide of the RYR.
  • Clinical and Translational Updates

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