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CHO/Human PD-L1 Stable Cell Line (Medium Expression) Development Service

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  • Description
    The CHO/ Human PD-L1 Stable Cell Line was engineered to express full length human PD-L1 receptor (Gene ID: 29126), with different levels of PD-L1 expression (High, Medium, Low), which can be used to mimic cancer target cells with various PD-L1 expression levels. When co-cultured with Human 4-1BB HEK293 Reporter Cell and anti-human 4-1BB/PD-L1 BsAb, the anti-human 4-1BB/PD-L1 BsAb can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.
  • Application

    • Useful for cell-based PD-L1 binding assay

    • Useful for PD-L1-mediated crosslinking in reporter gene assay

     PD-L1 Assay Principles

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    F-12K+ 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 PD-L1 FACS

Expression analysis of human PD-L1 on CHO/ Human PD-L1 Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human PD-L1 antibody was performed on CHO/Human PD-L1 Stable Cell Line with different expression levels: CHO/Human PD-L1 Stable Cell Line (Low Expression); CHO/Human PD-L1 Stable Cell Line (Medium Expression); CHO/Human PD-L1 Stable Cell Line (High Expression).

Application
 PD-L1 APPLICATION

Bioactivity analysis of anti-human 4-1BB/PD-L1 BsAb through CHO/Human PD-L1 Stable Cell Line (Medium Expression) crosslinking to test whether in a PD-L1-dependent manner to strengthen the agonistic activity.
The EC50 of anti-human 4-1BB/PD-L1 BsAb antibody is approximately 0.033 μM through CHO/Human PD-L1 Stable Cell Line (Medium Expression) crosslinking.

Please contact us if you are interested in related cell pool service.

  • Background
    Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €3540.00

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Drug Development Status

  • Number of Launched Drugs:7 Details
  • Number of Drugs in Clinical Trials:156 Details
  • Latest Research Phase:Phase 2 Clinical

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