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Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
AY63
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Tag
A protein peptide with his tag.
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
| Application | Recommended Usage |
| Western Blot | 0.2-1 ug/mL |
| ELISA | 0.1-16 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Human IgG1(AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) captured on Protein G Chip can bind Rabbit Serum Albumin, His Tag (Cat. No. RSA-R52H6) with an affinity constant of 29.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
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