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Human IL-1 RI Protein, Fc Tag (MALS verified)

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  • Synonym
    IL-1 RI
  • Source
    Human IL-1 RI Protein, Fc Tag(ILI-H5253) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Lys 336 (Accession # P14778).
    Predicted N-terminus: Leu 18
  • Molecular Characterization
    IL-1 RI Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 63.1 kDa. The protein migrates as 75-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 RI SDS-PAGE

Human IL-1 RI Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-1 RI MALS images

The purity of Human IL-1 RI Protein, Fc Tag (Cat. No. ILI-H5253) is more than 90% and the molecular weight of this protein is around 145-175 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-1 RI ELISA

Immobilized Human IL-1 beta, His Tag (Cat. No. ILB-H51H3) at 5 μg/mL (100 μL/well) can bind Human IL-1 RI Protein, Fc Tag (Cat. No. ILI-H5253) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    IL1R1 also know as Interleukin-1 receptor type 1, is the receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €260.00

Price(EUR) : €1620.00

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