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Your Position: Accueil > Protein > IFN-alpha/beta R1 > IF1-H82E3

Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag™ (MALS & SPR verified)

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  • Synonym
    IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1
  • Source
    Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag(IF1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1).
    Predicted N-terminus: Lys 28
  • Molecular Characterization
    IFN-alpha/beta R1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 50 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IFN-alpha/beta R1 SDS-PAGE

Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
IFN-alpha/beta R1 MALS images

The purity of Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (Cat. No. IF1-H82E3) is more than 90% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.

Bioactivity-SPR
 IFN-alpha/beta R1 SPR

Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (Cat. No. IF1-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 0.177 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Interferon alpha/beta receptor 1 ( IFNAR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFNAR, which belongs to the type II cytokine receptor family. IFNAR1 /IFNAR contains four fibronectin type-III domains. IFNAR1 associates with IFNAR2 to form the type I interferon receptor. IFNAR1 is receptor for interferons alpha and beta. IFNAR1 can transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €1390.00

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  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Approved

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