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Monoclonal Anti-Monkeypox-E8L Antibody, Human IgG1 (1C12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
1C12
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
Recombinant Monkeypox virus (strain Zaire-96-I-16) E8 derived from human 293 cells.
This product is a specific antibody specifically reacts with E8L (MPXV).
| Application | Recommended Usage |
| ELISA | 0.1-75 ng/mL |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-Monkeypox-E8L Antibody, Human IgG1 (1C12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Monoclonal Anti-Monkeypox-E8L Antibody, Human IgG1 (1C12) (Cat. No. E8L-M573) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Immobilized Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag (Cat. No. E8L-M52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-E8L Antibody, Human IgG1 (1C12) (Cat. No. E8L-M573) with a linear range of 0.1-2 ng/mL (QC tested).
Monoclonal Anti-Monkeypox-E8L Antibody, Human IgG1 (1C12) (Cat. No. E8L-M573) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag (Cat. No. E8L-M52H3) with an affinity constant of 0.119 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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