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Human IL-2 (N108D, C145A) & TSG-6 Protein, His Tag (MALS verified)

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  • Synonym
    IL-2 & TSG-6
  • Source
    Human IL-2 (N108D, C145A) & TSG-6, His Tag(IL6-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 & Gly 36 - Asn 129 (Accession # P60568-1 (N108D, C145A) & P98066-1).
    Predicted N-terminus: Ala 21
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 28.3 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-2 & TSG-6 SDS-PAGE

Human IL-2 (N108D, C145A) & TSG-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-2 & TSG-6 MALS images

The purity of Human IL-2 (N108D, C145A) & TSG-6, His Tag (Cat. No. IL6-H52H3) is more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-2 & TSG-6 ELISA

Immobilized Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) at 2 μg/mL (100 μL/well) can bind Human IL-2 (N108D, C145A) & TSG-6, His Tag (Cat. No. IL6-H52H3) with a linear range of 5-40 ng/mL (QC tested).

  • Background
    Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €450.00

Price(EUR) : €3230.00

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