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Your Position: Accueil > Protein > HLA-A*0201 & B2M & p53 (HMTEVVRRC) > HLP-H52H5

Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein (MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*0201 & B2M & p53 (HMTEVVRRC)
  • Source
    Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein(HLP-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & HMTEVVRRC peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & HMTEVVRRC).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein is assembled by biotinylated monomer (HLP-H82E4) and streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa, 13 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0201 & B2M & p53 (HMTEVVRRC) SDS-PAGE

Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0201 & B2M & p53 (HMTEVVRRC) MALS images

The purity of Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein (Cat. No. HLP-H52H5) is more than 90% and the molecular weight of this protein is around 255-295 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0201 & B2M & p53 (HMTEVVRRC) ELISA

Immobilized Anti-HLA class I Antibody, Human IgG1 (W6/32) at 1 μg/mL (100 μL/well) can bind Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein (Cat. No. HLP-H52H5) with a linear range of 0.5-16 ng/mL (QC tested).

  • Background
    TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (HMTEVVRRC) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HMTEVVRRC peptide of the p53.
  • Clinical and Translational Updates

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Price(EUR) : €280.00

Price(EUR) : €1780.00

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