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Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (MALS & SPR verified)

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  • Source
    Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (NEE-V5245) is expressed from human 293 cells (HEK293). It contains AA Tyr 33 - Leu 466 (Accession # EPI_ISL_983345, GISAID).
    Predicted N-terminus: His
  • Molecular Characterization
    Neuraminidase/NA (Influenza Virus) Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 55.7 kDa. The protein migrates as 65-80 kDa and 140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Neuraminidase/NA (Influenza Virus) SDS-PAGE

Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Neuraminidase/NA (Influenza Virus) MALS images

The purity of Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) is more than 85% and the molecular weight of this protein is around 260-290 kDa verified by SEC-MALS.

Bioactivity-SPR
 Neuraminidase/NA (Influenza Virus) SPR

a-Neu5Ac-PAA-biotin immobilized on SA Chip can bind Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) with an affinity constant of 59.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).

 Neuraminidase/NA (Influenza Virus) SPR

Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) immobilized on CM5 Chip can bind N-Acetylneuraminic Acid (NANA,Neu5Ac) with an affinity constant of 0.518 mM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €350.00

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