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Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
55F8
Human IgG1 | Human Kappa
Acridine ester
Recombinant Monoclonal
Tag
A protein peptide with his tag.
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable at 37°C for 48 hours.
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable after freezing and thawing 3 times.
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