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Use the kit after you extract host cell DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit Ⅱ (Magnetic Beads) (Cat. No. OPA-R024) in combination.
The kit is used for quantitative detection of residual HEK293 DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the HEK293 resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 5 (30 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of HEK293 genomic DNA (included in the kit) in the presence of CHO DNA and E. coli DNA.
| ID | Components | Size |
| OPA-R006-01 | 2×qPCR Master Mix | 1.0 mL×2 |
| OPA-R006-02 | HEK293 Primer & Probe Mix | 700 μL |
| OPA-R006-03 | HEK293 DNA Control (3 ng/μL) | 100 μL |
| OPA-R006-04 | Dilution Buffer | 1.5 mL×3 |
| OPA-R006-05 | DNase/RNase-Free Water | 1.0 mL |
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