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Biotinylated Mouse CD206 / MMR Protein, His,Avitag™

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  • Synonym
    MMR,CD206,hMR,MRC1,CLEC13D,CLEC13DL,MRC1L1
  • Source
    Biotinylated Mouse CD206 Protein, His,Avitag(CD6-M82E8) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Ala 1388 (Accession # Q61830).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    CD206 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 159.0 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD206 SDS-PAGE

Biotinylated Mouse CD206 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
CD206 SEC-HPLC

The purity of Biotinylated Mouse CD206 Protein, His,Avitag (Cat. No. CD6-M82E8) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 CD206 ELISA

Immobilized Mannan from Saccharomyces cerevisiae at 20 μg/mL (100 μL/well) can bind Biotinylated Mouse CD206 Protein, His,Avitag (Cat. No. CD6-M82E8) with a linear range of 0.078-0.625 μg/mL (QC tested).

  • Background
    Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. Mrc1 and its vertebrate homologue Claspin serve as a mediator for replication stress checkpoint signaling, receiving the signal from Mec1/Rad3/ATR sensor kinase and transmitting it to the effector Rad53/Cds1/Chk1 kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €350.00

Price(EUR) : €1540.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Approved

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