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Use the kit after you extract residual DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) in combination.
The kit is used for quantitative detection of residual E1A DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the E1A resDNA Quantitative Kit. (A) Typical analysis results obtained with Standard 1 (4×10⁶ copies/µL) to 6 (4×10 copies/µL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
To evaluate the specificity of assay using E1A resDNA Quantitation Kit, no template samples spiked with unrelated DNA were tested. Results shown in the following Table. Unrelated DNA (E. coli, CHO, Vero, and Pichia yeast) were not detected in assay, and the value of MDCK was outside the range of standard curve (4×10⁶ copies/µL to 4×10 copies/µL).
To evaluate the recovery of assay using E1A resDNA Quantitation Kit, three different concentrations of DNA samples were tested. Results shown in the following Table. All samples had recoveries between 95%-104%.
| ID | Components | Size |
| OPA-R008-01 | E1A Primer & Probe Mix | 550 μL |
| OPA-R008-02 | 2×qPCR Master Mix | 1.6 mL |
| OPA-R008-03 | Linearize DNA Control 4×10⁸ copies/μL | 50 μL |
| OPA-R008-04 | DNA Dilution Buffer | 1.5 mL×3 |
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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