0
Il n'y a pas de produit dans le panier !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9

Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVIL) Complex Protein (Monomer, MALS verified)

Order Now

  • Synonym
    HLA-E*0103 & B2M & CMV UL40 (VMAPRTVIL)
  • Source
    Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVIL) Complex Protein(HL0-H82E6)  is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVIL peptide (Accession # P13747  (HLA-E*01:03) & P61769-1  (B2M) & VMAPRTVIL).
    Predicted N-terminus: Gly 22 & Ile 21
  • Molecular Characterization

    Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVIL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.2 kDa and 13.7 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4  with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
HLA-E*0103 & B2M & CMV UL40 (VMAPRTVIL) SDS-PAGE

Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVIL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-E*0103 & B2M & CMV UL40 (VMAPRTVIL) MALS images

The purity of Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVIL) Complex Protein (Cat. No. HL0-H82E6) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

  • Background
    HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
  • Clinical and Translational Updates

Comments (0)


ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €120.00

Price(EUR) : €690.00

Recommended Products
Plaque enduite de streptavidine

Price(EUR) : €30.00

Price(EUR) : €140.00

Streptavidine-HRP

Price(EUR) : €50.00

Price(EUR) : €120.00

Billes magnétiques™ Streptavidine

Price(EUR) : €140.00

Price(EUR) : €230.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop

Laisser un message