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HEK293/Human PD-1 Stable Cell Line

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  • Description
    The HEK293/Human PD-1 Stable Cell Line was engineered to express human PD-1 (AA sequence : Leu 25 - Leu 288, Protein Acc.# : NP_005009.2). Surface expression of Human PD-1 was confirmed by flow cytometry.
  • Application

    • Useful for cell-based PD-1 binding assay.

  • Growth Properties
    Adherent
  • Selection Marker
    Hygromycin B(300 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    10% DMSO + 90% FBS
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 PD-1 FACS

Fig. FACS analysis of PD-1 on HEK293/Human PD-1 Stable Cell Line.
FACS assay shows that Human PD-L1 Protein, Mouse IgG1 Fc Tag can bind to HEK293/Human PD-1 Stable Cell Line. HEK293/Human PD-1 Stable Cell Line was red line, negative control HEK293 cells was grey line(QC tested).

Please contact us if you are interested in related cell pool service.

  • Background
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €3540.00

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Drug Development Status

  • Number of Launched Drugs:16 Details
  • Number of Drugs in Clinical Trials:150 Details
  • Latest Research Phase:Approved

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