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Fluorescent Human GPRC5D Full Length Protein (VLP)

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  • Synonym
    GPRC5D
  • Source
    Fluorescent Human GPRC5D Full Length Protein (VLP)(GPD-HF2G5) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Val 345 (Accession # Q9NZD1-1).
    Predicted N-terminus: Asp
  • Molecular Characterization

    This protein carries a GFP tag.

    The protein has a calculated MW of 42.1 kDa | 83.1 kDa.

    Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • Conjugate

    GFP

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 530 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

    Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-NF2P4) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 GPRC5D ELISA

Immobilized Fluorescent Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-HF2G5) at 2 μg/mL (100 μL/well) can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.5-4 ng/mL (QC tested).

Identity-DLS
 GPRC5D DLS

The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

  • Background
    The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. (provided by RefSeq, Jul 2008)
  • Clinical and Translational Updates

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