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The kit is developed for quantitative detection of natural and recombinant human EGF in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | EGF |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | <35pg/mL |
| Range | 62.5 pg/mL-8000 pg/mL |
| Assay Time | 2 hr 45 min |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of EGF is less than 35 pg/mL.
Ten replicates of each of 3 samples containing different EGF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of EGF were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant EGF was spiked into 3 human serum samples, and then analyzed. The average recovery of EGF for serum samples is 105%.
| ID | Components | Size |
| CEA080-C01 | Pre-coated Anti-EGF Antibody Microplate | 1 plate |
| CEA080-C02 | Human EGF Standard | 7.5 ug×2 |
| CEA080-C03 | Biotin-Anti-EGF Antibody Con. Solution | 100 μL |
| CEA080-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA080-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA080-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA080-C07 | 20× Washing Buffer | 50 mL |
| CEA080-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA080-C09 | Substrate Solution | 12 mL |
| CEA080-C10 | Stop Solution | 6 mL |
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