Limited Edition Golden Llama is here! Check out how you can get one. Limited Edition Golden Llama is here! Check out how you can get one.
Offering SPR-BLI Services - Proteins provided for free! Get your ComboX free sample to test now!
Time Limited Offer: Welcome Gift for New Customers ! Shipping Price Reduction for EU Regions
Purity (SDS PAGE) | > 95% |
Mycoplasma Test | Negative |
Sterility Test | Negative |
Integrin Binding Assay | 0.5 nM < KD < 5 nM |
Endotoxin Test | < 0.01 EU per μg |
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The cell proliferation ability of Human Laminin 521 Protein, Research Grade (Cat. No. LA5-H5215) measured by the CTG method is better than Basement Membrane Extract (Company C) and Vitronectin even in different working concentrations (5 μg/mL, 10 μg/mL).
Human Laminin 521 Protein, Research Grade (Cat. No. LA5-H5215) could maintain the stemness of iPSC at least Passage 5. Immunofluorescent staining indicated that the iPSCs expressed high levels of pluripotency associated markers Sox2, TRA-1-60, SSEA4 and OCT4.
Human Laminin 521 Protein, Research Grade (Cat. No. LA5-H5215) could maintain the stemness of iPSC at least Passage 5. FACS data indicated that the iPSCs expressed high levels of pluripotency associated markers OCT4 and SOX2.
Normal karyotype (46, XX) was found in hiPSCs with Human Laminin 521 Protein, Research Grade (Cat. No. LA5-H5215) coating after 10 passages.
Loaded Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) on HIS1K Biosensor, can bind Human Laminin 521 Protein, Research Grade (Cat. No. LA5-H5215) with an affinity constant between 0.50 nM - 5.00 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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