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Biotinylated Human Activin RIIA Protein, His,Avitag™

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  • Synonym
    Activin receptor type IIA,ACTRIIA
  • Source
    Biotinylated Human Activin RIIA Protein, His,Avitag(ACA-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 135 (Accession # P27037-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Activin RIIA Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 17 kDa. The protein migrates as 28-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Activin RIIA SDS-PAGE

Biotinylated Human Activin RIIA Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Activin RIIA ELISA

Immobilized Human Latent Activin A, His Tag (Cat. No. ACA-H424x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIA Protein, His,Avitag (Cat. No. ACA-H82Q3) with a linear range of 0.078-1.25 μg/mL (QC tested).

  • Background
    Activin receptor type-2A (ACVR2A) is also known as Activin receptor type IIA, ACTR-IIA, ACTRIIA and ACVR2,which is single-pass type I membrane protein. ACVR2A belongs to the protein kinase superfamily,TKL Ser/Thr protein kinase family and TGFB receptor subfamily.On ligand binding,ACVR2A can forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators.ACVR2A is Receptor for activin A, activin B and inhibin A as well. Several type I I activin receptors have been identified and bind to different activins with different affinities.
  • Clinical and Translational Updates

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  • Latest Research Phase:Phase 2 Clinical

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