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Your Position: Accueil > Protein > Integrin alpha V beta 6 > IT6-M52E3

Mouse & Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free (MALS verified)

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  • Synonym
    Integrin alpha V beta 6,ITGAV&ITGB6
  • Source
    Mouse & Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free(IT6-M52E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 & Gly 22 - Asn 707 (Accession # P43406-1 & AAI21179.1).
    Predicted N-terminus: Phe 31 & Gly 22
  • Molecular Characterization
    Integrin alpha V beta 6 Structure

    The protein has a calculated MW of 112.5 kDa & 79.6 kDa. The protein migrates as 130-150 kDa and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha V beta 6 SDS-PAGE

Mouse & Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Integrin alpha V beta 6 MALS images

The purity of Mouse & Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-M52E3) is more than 85% and the molecular weight of this protein is around 210-260 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Integrin alpha V beta 6 ELISA

Immobilized Mouse & Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-M52E3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) with a linear range of 0.2-10 ng/mL (QC tested).

  • Background
    Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €410.00

Price(EUR) : €2910.00

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  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Phase 3 Clinical

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