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| ID | Components | Size |
| RAS210-C01 | Pre-coated Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody Microplate | 1 plate |
| RAS210-C02 | HA (Influenza A/Darwin/9/2021 (H3N2)) Standard | 20 μg |
| RAS210-C03 | Biotin-Anti-HA(Influenza A (H3N2)) Antibody | 20 μg |
| RAS210-C04 | Streptavidin-HRP | 10 μg |
| RAS210-C05 | 10×Washing Buffer | 50 mL |
| RAS210-C06 | 2×Dilution Buffer | 50 mL |
| RAS210-C07 | Substrate Solution | 12 mL |
| RAS210-C08 | Stop Solution | 7 mL |
This kit is developed for specific quantitative detection of Influenza A/Darwin/9/2021 virus HA protein in samples.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the HA (Influenza A/Darwin/9/2021 (H3N2)) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the Secondary antibody biotin-Anti-HA (Influenza A(H3N2)) Antibody diluted by Dilution Buffer to the plate.
d) Add the diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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