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> Contrôles d'isotype IgG
Les anticorps sont devenus un domaine important dans le développement de la biomédecine. Les anticorps de contrôle d'isotype dans le développement de médicaments anticorps sont de la même classe et du même sous-type que l'anticorps primaire développé contre la cible, mais ne se lient pas spécifiquement à une cible connue ou potentielle. L'objectif de l'utilisation du contrôle d'isotype est de confirmer que la liaison ou l'effet de l'anticorps primaire est spécifique, excluant les interactions non spécifiques avec les protéines du récepteur Fc ou avec d'autres protéines. Parallèlement, le contrôle de l'isotype peut également être utilisé comme un anticorps qui entre en compétition pour la liaison aux protéines des récepteurs Fc, ce qui est apparenté à l'anticorps bloquant fonctionnel qui remplit la même fonction, ce qui facilite l'évaluation efficace et raisonnable de l'efficacité des anticorps. Le fond non spécifique est une variable importante qui doit être contrôlée. La mise en place de l'anticorps témoin isotype correspondant pour détecter les liaisons non spécifiques peut réduire la génération de résultats faussement positifs et évaluer avec précision les facteurs d'influence possibles dans le processus de développement du médicament.
ACROBiosystems a développé une série de contrôles d'isotype IgG avec des espèces humaines et murines et différents sous-types, qui peuvent être utilisés comme anticorps de contrôle d'isotype dans diverses applications telles que ELISA, western blot, immunofluorescence, immunohistochimie (IHC) et cytométrie de flux (FACS), etc.
| Molecule | Cat. No. | Species | Product Description | Preorder/Order |
|---|---|---|---|---|
| IgG Control | DNP-M2 | Human | Human IgG1 Kappa Isotype Control (mAb, carrier free, MALS verified) | |
| DNP-BM2 | Biotinylated Human IgG1 Kappa Isotype Control (Biotin, mAb, carrier free, MALS verified) | |||
| DNP-MB273 | Human IgG1 (N297A) Kappa Isotype Control (mAb, carrier free, MALS verified) | |||
| DNP-M915 | Human IgG3 Kappa Isotype Control (mAb, carrier free) | |||
| DNP-M3 | Human IgG4 Kappa Isotype Control (mAb, carrier free, MALS verified) | |||
| DNP-FM2 | FITC-Labeled Human IgG1 Kappa Isotype Control (FITC, mAb, carrier free) | |||
| DNP-M1 | Mouse | Mouse IgG1 Kappa Isotype Control (mAb, Carrier free, MALS verified) | ||
| DNP-BM1 | Biotinylated Mouse IgG1 Isotype Control (Biotin, mAb, carrier free, MALS verified) | |||
| DNP-M1A1 | Mouse IgG1 kappa Isotype Control | |||
| DNP-BM190 | Biotinylated Mouse IgG1 kappa Isotype Control | |||
| DNP-FM1 | FITC-Labeled Mouse IgG1 Isotype Control (FITC, mAb, carrier free) | |||
| DNP-FM1A1 | FITC-Labeled Mouse IgG1 Isotype Control | |||
| DNP-PM1 | PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Site-specific conjugation) |
Human IgG1 (N297A) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The purity of Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG4 Kappa Isotype Control (mAb) (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
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