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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.0 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Mesothelin (296-580), His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) is more than 90% and the molecular weight of this protein is around 32-47 kDa verified by SEC-MALS.
2e5 of Anti-MSLN CAR-293 cells were stained with 100 μL of 1 μg/mL of Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) and negative control protein respectively, washed and then followed by His Tag PE-conjugated Antibody and analyzed with FACS (QC tested).
Immobilized Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) at 0.5 μg/mL (100 μL/well) can bind Anti-MSLN MAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
Loaded Monoclonal Anti-Human MSLN Antibody, Human IgG1 on Protein A Biosensor, can bind Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) with an affinity constant of 3.57 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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