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Your Position: Accueil > Protein > Siglec-10 > SI0-H82F6

Biotinylated Human Siglec-10 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
  • Source
    Biotinylated Human Siglec-10, Fc,Avitag(SI0-H82F6) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
    Predicted N-terminus: Met 17
  • Molecular Characterization
    Siglec-10 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 86.4 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Siglec-10 SDS-PAGE

Biotinylated Human Siglec-10, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Siglec-10 MALS images

The purity of Biotinylated Human Siglec-10, Fc,Avitag (Cat. No. SI0-H82F6) is more than 90% and the molecular weight of this protein is around 176-216 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Siglec-10 ELISA

Immobilized Biotinylated Human Siglec-10, Fc,Avitag (Cat. No. SI0-H82F6) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-20 ng/mL (QC tested).

  • Background
    The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €1540.00

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  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Phase 3 Clinical

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