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Your Position: Accueil > Protein > IL-15 R alpha > ILA-HF255

FITC-Labeled Human IL-15 R alpha / CD215 Protein, Fc Tag (MALS verified)

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  • Synonym
    IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha
  • Source
    FITC-Labeled Human IL-15 R alpha, Fc Tag(ILA-HF255) is expressed from human 293 cells (HEK293). It contains AA Ile 31-Thr 205 (Accession # Q13261-1).
    Predicted N-terminus: Ile 31
  • Molecular Characterization
    IL-15 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 44.8 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-15 R alpha SDS-PAGE

FITC-Labeled Human IL-15 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-15 R alpha MALS images

The purity of FITC-Labeled Human IL-15 R alpha, Fc Tag (Cat. No. ILA-HF255) is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-15 R alpha ELISA

Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-15 R alpha, Fc Tag (Cat. No. ILA-HF255) with a linear range of 0.078-5 μg/mL (QC tested).

  • Background
    IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €320.00

Price(EUR) : €2200.00

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